重組小鼠白介素-19（rmIL-19）
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- 更新時間： 2024-05-23
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產(chǎn)品介紹
| CYT-411 | 重組小鼠白介素-19（rmIL-19） | 2μg/10μg/1mg | Prospec |
| Background : | |
| Interleukin-19 （IL-19） is a novel cytokine that was initially identified during a sequence data base search aimed at finding potential Interleukin-10 homologs. Interleukin-19 shares 21% amino acid identity with IL-10. The exon/intron structure of IL-19 is similar to that of the human IL-10 gene, comprising five exons and four introns within the coding region of the IL-19 cDNA. IL-19 shares a receptor complex with IL-20, indicating that the biological activities of these two cytokines overlap and that both may play an important role in regulating development and proper functioning of the skin. Unlike IL-10, which forms an intercalated dimer, the molecule of IL-19 is a monomer made of seven amphipathic helices, A-G, creating a unique helical bundle. | |
 | Description : |
| Recombinant Mouse IL-19 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids and having a molecular mass of 17722 Dalton. Recombinant Human IL-19 is purified by proprietary chromatographic techniques. | |
| Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| Lyophilized from a concentrated （1mg/ml） solution in water containing no additives. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized Recombinant IL-19 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized Mouse Interleukin-19 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant Mouse IL-19 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Arg-Cys. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| ProSpec’s Recombinant Mouse IL-19 is fully biologically active when compared to standard. The biological activity was determined by measuring the dose-dependent activation of STAT3 in human epidermal keratinocytes （HEK2a cells）. Significant STAT3 activation is observed with >100ng/mL of recombinant mouse IL-19. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of Mouse IL-19. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IL-19 as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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