重組大鼠白介素-6（rrIL-6）
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- 更新時間： 2024-05-23
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產品介紹
| CYT-388 | 重組大鼠白介素-6（rrIL-6） | 2μg/10μg/1mg | Prospec |
| Background: | |
| Interleukin （IL）-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells.has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. | |
 | Description : |
| Recombinant Rat IL-6 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21732 Dalton. Recombinant Rat IL-6 is purified by proprietary chromatographic techniques. | |
| Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| The protein was lyophilized without any additives. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized Recombinant Rat IL-6 in 50mM acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized Recombinant Rat IL-6 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Rat IL-6 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 95.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| ProSpec’s Recombinant Rat IL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.01 ng/ml, corresponding to a Specific Activity of 1 x 108 IU/mg. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of Rat IL-6. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
佰易聚生物商城提供美國光譜醫學透析袋，wako生化試劑，amresco生化試劑，sigma生化試劑，優質的分子生物學試劑和試劑盒、培養基本和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄 www.ebioeasy.com或者,，洽談選購！
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