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重組人白介素-17 (IL-17)

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CYT-250重組人白介素-17 (IL-17) 5μg/25μg/1mg Prospec

 

 

Description :
 Recombinant Human IL-17A produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing a total of 264 amino acids and having a molecular mass of 30,250 Dalton.
Recombinant IL-17 is purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 Lyophilized from a concentrated (1mg/ml) solution containing no additives.

 
Solubility:
 It is recommended to reconstitute the lyophilized Human IL-17 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized Recombinant IL-17A although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution IL-17 should be stored at 40C between 2-7 days and for future use below -180C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
 Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be
Gly-Ile-Thr-Ile-Pro.

 
Biological Activity:
 The ED50 as determined by the dose-dependant induction of IL-6 in primary human foreskin fibroblasts was found to be 2 ng/mL, corresponding to a specific activity of 5 x 105 Units/mg.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of Recombinant Human IL-17.

 
Protein content:
 Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.13 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-17 as a Reference Standard.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

 


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