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CYT-283

重組人干擾素-α 1b （rHuIFN-alpha 1b）

20μg/100μg/1mg

Prospec

Background:
	At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
	Description :
	Recombinant Human Interferon alpha-1b produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19392 Dalton. The Interferon-alpha 1b gene was obtained from human leukocytes. The IFN-alpha-1b is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	Lyophilized from a （1mg/ml） solution in containing 20mg HSA and 7.5mg sodium chloride.
	Solubility:
	It is recommended to reconstitute the lyophilized IFN alpha-1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized IFN-alpha-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IFN-alpha-1b should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Glu.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec's Interferon-alpha 1b is fully biologically active when compared to WHO standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 1 x 10⁸ IU/ mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of IFN-alpha 1b.
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm . 2. Analysis by RP-HPLC, using a standard solution of IFN-alpha 1b as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Latest Publications:

1. [Gene modification and high prokaryotic expression of porcine interferon alpha-1]

Sheng Wu Gong Cheng Xue Bao 2004 Mar;20（2）:291-4

2. [Translational control of human interferon alpha 1 gene expression in E.coli]

Zhongh ua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2002 Dec;16（4）:315-8

3. Differential effects of angiostatin, endostatin and interferon-alpha（1） gene transfer on in vivo growth of human breast cancer cells.

Gene Ther 2002 Jul;9（13）:867-78

4. High-yield expression and purification of human interferon alpha-1 in Pichia pastoris.

Protein Expr Purif 2001 Aug;22（3）:381-7

5. Unusual effect of clusters of rare arginine （AGG） codons on the expression of human interferon alpha 1 gene in Escherichia coli.

Int J Biochem Cell Biol 1997 Apr;29（4）:659-66

6. Extracellular matrix remodelling in a murine mammary adenocarcinoma transfected with the interferon-alpha 1 gene.

J Pathol 1997 Jan;181（1）:116-23

佰易聚生物商城提供美國光譜醫學透析袋，wako生化試劑，amresco生化試劑，sigma生化試劑，優質的分子生物學試劑和試劑盒、培養基本和培養耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現貨火爆*,歡迎登錄 www.ebioeasy.com或者,，洽談選購！

在線咨詢

上一篇：重組人干擾素-β 1a （rHuIFN-β 1a）
下一篇：重組人干擾素-γ（rHuIFN-γ）

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