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重組人成纖維細胞生長因子-22(FGF-22)

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CYT-428重組人成纖維細胞生長因子-22(FGF-22) 2μg/10μg/1mg Prospec

 

 

Background :
 
 
Description :
 Recombinant Human FGF-22 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 148 amino acids and having a molecular mass of 17173 Dalton.
Recombinant FGF-22 is purified by chromatographic techniques.
 
Physical Appearance:
 Sterile Filtered white lyophilized powder.
 
Formulation:
 The sterile protein powder is lyophilized with no additives.
 
Solubility:
 It is recommended to reconstitute the lyophilized Recombinant Human FGF-22 sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
 
Stability:
 Lyophilized rHuFGF-22 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution rHuFGF-22 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
 
Purity:
 Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be
Thr-Pro-Ser-Ala-Ser.
 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
 
Biological Activity:
 Measured in a cell proliferation assay using IEC6 cell line (20ng/ml can stimulate the cell proliferation of IEC6 cell line).
 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of Recombinant Human FGF-22.
 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


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