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CYT-217

重組人表皮生長因子（rHuEGF）

100μg/500μg

Prospec

Background:
	A neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system.
	Description :
	Recombinant Human Epidermal Growth Factor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton. The EGF is purified by proprietary chromatographic techniques.
	Physical Appearance:
	Sterile Filtered White lyophilized （freeze-dried） powder.
	Formulation:
	Recombinant EGF was lyophilized from a concentrated （1mg/ml） solution with no additives.
	Solubility:
	It is recommended to reconstitute the lyophilized EGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
	Stability:
	Lyophilized EGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution EGF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles.
	Purity:
	Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
	Amino acid sequence:
	The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu, which agrees with the sequence of native human EGF. N-terminal methionine has been compley removed enzymatically.
	Dimers and aggregates:
	Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
	Biological Activity:
	ProSpec's recombinant Human Epidermal Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells （measured by 3H-thymidine uptake） is less then 0.1 ng/ml corresponding to a specific activity of 1 x 10⁷ Units/mg.
	Endotoxin:
	Less than 0.1 ng/µg （IEU/µg） of recombinant EGF .
	Protein content:
	Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of human EGF as a Reference Standard.
	Usage:
	Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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