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重組人前骨形態(tài)發(fā)生蛋白-2(rHuProBMP-2)

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CYT-427重組人前骨形態(tài)發(fā)生蛋白-2(rHuProBMP-2) 2μg/10μg/100ug Prospec

 

 

Description:
 Recombinant Human ProBMP-2 is the precursor of BMP-2 which has 3 intramolecular cystein bridges is a homodimer, non-glycosylated, Polypeptide chain containing 376 amino acids and having a molecular mass of 85,726 Dalton.
Recombinant ProBMP-2 is purified by proprietary chromatographic techniques.

 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
 The protein was lyophilized from a concentrated (0.1mg/ml) sterile solution containing 10mM HCL pH=2.

 
Solubility:
 It is recommended to reconstitute the lyophilized Recombinant Human ProBMP-2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
 Lyophilized ProBMP-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant ProBMP-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
 Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

 
Amino acid sequence:
 GAAGLVPELGRRKFAAASSGRPSSQPSDEVLSEFELRLLSMFGLKQRPTPSRDAVVPPYMLDLYR
RHSGUPGSPAPDHRLERAASRANTVRSFHHEESLEELPETSGKTTRRFFFNLSSIPTEEFITSAELQ
VFREQMQDALGNNSSFHHRINIYEIIKPATANSKFPVTRLLDTRLVNQNASRWESFDVTPAVMRW
TAQGHANHGFVVEVAHLEEKQGVSKRHVRISRSLHQDEHSWSQIRPLLVTFGHDJKJHPLHKREK
RQAHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAYVQ
TLVNSVNSKIPKACCVPSAISMLYLDENKVVLKNYQDMVVEGCGCR

 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
 The ED50 as determined by the stimulating effect on the migration of C3T3 cells and was found active.

 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of ProBMP-2.

 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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