重組人前骨形態(tài)發(fā)生蛋白-2(rHuProBMP-2)
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- 更新時間: 2024-05-23
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產(chǎn)品介紹
CYT-427 | 重組人前骨形態(tài)發(fā)生蛋白-2(rHuProBMP-2) | 2μg/10μg/100ug | Prospec |
Description: | |
Recombinant Human ProBMP-2 is the precursor of BMP-2 which has 3 intramolecular cystein bridges is a homodimer, non-glycosylated, Polypeptide chain containing 376 amino acids and having a molecular mass of 85,726 Dalton. Recombinant ProBMP-2 is purified by proprietary chromatographic techniques. | |
Physical Appearance: | |
Sterile Filtered White lyophilized (freeze-dried) powder. | |
Formulation: | |
The protein was lyophilized from a concentrated (0.1mg/ml) sterile solution containing 10mM HCL pH=2. | |
Solubility: | |
It is recommended to reconstitute the lyophilized Recombinant Human ProBMP-2 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
Stability: | |
Lyophilized ProBMP-2 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant ProBMP-2 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. | |
Purity: | |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
Amino acid sequence: | |
GAAGLVPELGRRKFAAASSGRPSSQPSDEVLSEFELRLLSMFGLKQRPTPSRDAVVPPYMLDLYR RHSGUPGSPAPDHRLERAASRANTVRSFHHEESLEELPETSGKTTRRFFFNLSSIPTEEFITSAELQ VFREQMQDALGNNSSFHHRINIYEIIKPATANSKFPVTRLLDTRLVNQNASRWESFDVTPAVMRW TAQGHANHGFVVEVAHLEEKQGVSKRHVRISRSLHQDEHSWSQIRPLLVTFGHDJKJHPLHKREK RQAHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAYVQ TLVNSVNSKIPKACCVPSAISMLYLDENKVVLKNYQDMVVEGCGCR | |
Dimers and aggregates: | |
Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
Biological Activity: | |
The ED50 as determined by the stimulating effect on the migration of C3T3 cells and was found active. | |
Endotoxin: | |
Less than 0.1 ng/µg (IEU/µg) of ProBMP-2. | |
Usage: | |
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
佰易聚生物商城提供美國光譜醫(yī)學透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優(yōu)質的分子生物學試劑和試劑盒、培養(yǎng)基本和培養(yǎng)耗材、細胞因子和細胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購!
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