產品介紹
| CYT-407 | 重組人神經調節素-1（ rHuNRG1） | 10μg/50μg/1mg | | Prospec |
| Background : | | | Neuregulin is a signaling protein for ErbB2/ErbB4 receptor heterodimers on the cardiac muscle cells, playing an important role in heart structure and function through inducing ErbB2/ErbB4 receptor phosphorylation and cardiomyocyte differentiation. Research on molecular level discovered that recombinant neuregulin could make disturbed myocardial cell structure into order and strengthen the connection between myocardial cells by intercalated discs re-organization. Pharmacodynamic experiments in animals showed that recombinant neuregulin （NRG1） can reduce the degree of damage on myocardial cells caused by ischemia, hypoxia and viral infection.
|  | Description : | | | Neuregulin is a signaling protein for ErbB2/ErbB4 receptor heterodimers on the cardiac muscle cells, playing an important role in heart structure and function through inducing ErbB2/ErbB4 receptor phosphorylation and cardiomyocyte differentiation. Research on molecular level discovered that recombinant neuregulin could make disturbed myocardial cell structure into order and strengthen the connection between myocardial cells by intercalated discs re-organization. Pharmacodynamic experiments in animals showed that recombinant neuregulin （NRG1） can reduce the degree of damage on myocardial cells caused by ischemia, hypoxia and viral infection. Recombinant Human Heregulin-b2 also called Neuroregulin-1 （Neu differentiation factor） produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 61 amino acids and having a total molecular mass of 7055Dalton. rHuNRG1 is purified by proprietary chromatographic techniques.
| | Physical Appearance: | | | Sterile Filtered White lyophilized （freeze-dried） powder.
| | Formulation: | | | Lyophilized from a 0.2μm filtered solution （0.25mg/ml） in 20mM PB, pH 7.0, containing 0.5%HSA and 2% mannitol.
| | Solubility: | | | It is recommended to reconstitute the lyophilized rHuNRG1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
| | Stability:
| | | Lyophilized Recombinant Human NRG1 although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution rHuNRG1 should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please avoid freeze-thaw cycles.
| | Purity: | | | Greater than 95.0% as determined by:
（a） Analysis by RP-HPLC.
（b） Anion-exchange FPLC.
（c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
| | Amino acid sequence: | | | SHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKAEELYQ
| | Dimers and aggregates: | | | Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
| | Biological Activity: | | | ProSpec’s Recombinant Human NRG1 is fully biologically active when compared to standard. The ED50, calculated by the dose-dependant stimulation of human MCF-7 cells is < 0.5ng/ml, corresponding to a specific activity of > 2 x 106 units/mg.
| | Endotoxin: | | | Less than 0.1 ng/µg （IEU/µg） of Recombinant NRG-1.
| | Protein content: | | | Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a standard solution of NRG1 as a Reference Standard.
| | Usage: | | | Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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| Latest Publications: | | | 1. An acute effect of neuregulin 1 beta to suppress alpha 7-containing nicotinic acetylcholine receptors in hippocampal interneurons.
J Neurosci 2006 Nov 1;26（44）:11295-303
2. Neuregulin-1/erbB-activation improves cardiac function and survival in models of ischemic, dilated, and viral cardiomyopathy.
J Am Coll Cardiol 2006 Oct 3;48（7）:1438-47
3. " To erb-B or not to erb-B... " Neuregulin-1/ErbB signaling in heart development and function.
J Mol Cell Cardiol 2006 Aug;41（2）:215-8
4. The N-terminal domains of neuregulin 1 confer signal attenuation.
J Biol Chem 2006 Sep 15;281（37）:27306-16
5. Altered neuregulin 1-erbB4 signaling contributes to NMDA receptor hypofunction in schizophrenia.
Nat Med 2006 Jul;12（7）:824-8
6. A novel missense mutation in the transmembrane domain of neuregulin 1 is associated with schizophrenia.
Biol Psychiatry 2006 Sep 15;60（6）:548-53 |
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