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Thermo Scientific AminoLink Plus Coupling Resin and Immobilization Kits use activated beaded agarose and a robust coupling chemistry to immobilize proteins and other ligands through primary amines (–NH2) to the resin.

Once an antibody or other ligand is immobilized, the prepared affinity resin can be used for a variety of affinity purification methods involving batch or column chromatography. The resin and linkage are stable in binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for at least 10 rounds of affinity purification.

Highlights:

• AminoLink Plus Coupling Resin – aldehyde-activated crosslinked 4% beaded agarose
• Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH2)
• Flexible coupling conditions – efficient (>85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent); regular (PBS, pH 7.2) and enhanced (citrate-carbonate, pH 10) coupling protocols provided
• Stable, permanent immobilization – Coupling reaction esults in stable, leak-resistant secondary amine bond between resin and ligand
• Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
• Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions
• Convenient kits and product sizes –choose 1- or 5-column kit containing complete sets of buffers, reagents and versatile spin/drip columns, or select bulk resin.