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PIERCE,N-Succinimidyl 3-(2-Pyridyldithio)Propionat

貨號:21857,名稱:N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP),品牌:PIERCE,規格:50 MG
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N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)介紹


Thermo Scientific BCA蛋白定量產品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。


N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)現貨供應



PIERCE

21857

N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)

50 MG

PIERCE

22122

Sulfo-SMCC, 1 g.

1 G

PIERCE

22305

MPBH: 4-(4-N-Maleimidophenyl)Butyric Acid Hydrazide Hydrochl

50 MG.


N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)品牌介紹


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N-Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)

僅用于科研


Thermo Scientific Pierce SPDP is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.


Thermo Scientific Pierce LC-SPDP is a long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls.

We offer three analogs of SPDP: the standard version (SPDP), a derivative with a longer spacer arm (LC-SPDP) and Sulfo-LC-SPDP, the sulfonated analog of LC-SPDP is available for applications that require a water-soluble, membrane-impermeable crosslinker. SPDP (N -Succinimidyl 3-(2-pyridyldithio)-propionate) and LC-SPDP (Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexanoate) are heterobifunctional, thiol-cleavable and membrane permeable crosslinkers. They each contain an amine-reactive N-hydroxysuccinimide (NHS) ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.

Highlights:

  • Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
  • Amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
  • Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
  • Cleavable crosslinker allows separation of crosslinked products
  • SPDP crosslinker is membrane permeable, so crosslinking can be carried out inside the cell


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