PIERCE，DISUCCINIMIDYL SUBERATE, （DSS）50 MG
- 型號(hào)： 21655
- 更新時(shí)間： 2024-05-31
- 上一個(gè)：21650PIERCE，Sulfo-LC-SPDP, sulfosuccinimidyl 6-（3'-（2 p
- 下一個(gè)：21667PIERCE，DIMETHYL PIMELIMIDATE. 2HCl（DMP）
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DISUCCINIMIDYL SUBERATE, （DSS）50 MG介紹
Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。
DISUCCINIMIDYL SUBERATE, （DSS）50 MG現(xiàn)貨供應(yīng)
PIERCE | 21655 | DISUCCINIMIDYL SUBERATE, （DSS）50 MG | 50 MG |
PIERCE | 21667 | DIMETHYL PIMELIMIDATE. 2HCl （DMP） | 1 GM |
PIERCE | 21857 | N-Succinimidyl 3-（2-Pyridyldithio）Propionate （SPDP） | 50 MG |
DISUCCINIMIDYL SUBERATE, （DSS）50 MG品牌介紹
美國(guó)Pierce公司（www.pierce.com）是蛋白質(zhì)化學(xué)、免疫學(xué)和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html
主要產(chǎn)品包括：
1）化學(xué)發(fā)光體和比色底物
2）分析試劑
3）熒光標(biāo)記探針
4）酶聯(lián)免疫分析試劑盒等
DISUCCINIMIDYL SUBERATE, （DSS）50 MG僅用于科研
Thermo Scientific Pierce Sulfo-LC-SPDP is a water-soluble, long-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable （reducible） disulfide bonds with cysteines.
Sulfo-LC-SPDP is Sulfosuccinimidyl 6-（3'-[2-pyridyldithio]-propionamido）hexanoate, a heterobifunctional, thiol-cleavable and membrane permeable crosslinker. It contains an amine-reactive N-hydroxysuccinimide （NHS） ester that will react with lysine residues to form a stable amide bond. The other end of the spacer arm is terminated in the pyridyl disulfide group that will react with sulfhydryls to form a reversible disulfide bond.
Highlights:
- Releases a detectable by-product when reacted with a free sulfhydryl group; by measuring the release of pyridine-2-thione at 343 nm, the reaction can be easily followed
- Water soluble, amine reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
- Cleavable crosslinker allows separation of crosslinked products
- Sulfo-LC-SPDP crosslinker is not membrane permeable, so crosslinking can be carried out on the cell surface